Εφαρμογές της αλληλουχίας RNA ενός κυττάρου στην ανακάλυψη και ανάπτυξη φαρμάκων
Date Issued
October 23, 2024
Type
Πτυχιακή Εργασία
Abstract
The advent of single-cell RNA sequencing (scRNA-seq) has revolutionised the field of
drug discovery and development, enabling high-resolution profiling of individual cells.
This thesis explores the applications of scRNA-seq in drug discovery and development,
focusing on its role in elucidating cellular heterogeneity, identifying novel therapeutic
targets and advancing pharmacogenomics. Chapter 1 provides an introduction to the
importance of molecular biology in drug discovery and the historical development of
sequencing technologies, leading to the advent of RNA sequencing and the subsequent
emergence of scRNA-seq. The aims and objectives of the thesis are outlined. Chapter
2 traces the evolution of DNA sequencing technologies, from Sanger sequencing to
next-generation sequencing, and discusses the transition from microarray technology
to RNA-Seq, which set the stage for scRNA-seq. Chapter 3 delves into the
fundamentals and technologies of single-cell analysis, including the various platforms
for single-cell analysis, methods for sampling and processing cells, and techniques for
single-cell surface functionalization and intracellular electrophysiology. Chapter 4
explores the contribution of scRNA-seq to drug discovery, focusing on its applications
in the study of cellular heterogeneity in oncology, the identification of novel therapeutic
targets and its implications for pharmacogenomics. Chapter 5 reviews computational
methods in drug design, including the modelling and simulation of structure-functional
targets and the use of algorithms and machine learning in the prediction of
pharmacological action. In conclusion, this thesis highlights the pivotal role of scRNAseq in the drug discovery and development revolution, paving the way for more targeted and personalized therapeutic interventions
Subjects